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	Provedor de dados Biological Sciences (2) Autor Amorim, Rodrigo César das Neves; Universidade Federal da Amazônia (1) Benevides, Norma Maria Barros; Universidade Federal do Ceará (1) Benevides, Norma Maria Barros; Universidade Federal do Ceará - UFC (1) Bessa, Érika Façanha; Universidade Federal do Ceará - UFC (1) Holanda, Márjory Lima; Universidade Federal do Maranhão (1) Mourão, Paulo Antônio de Souza; Universidade Federal do Rio de Janeiro (1) Queiroz, Ismael Nilo Lino de; Universidade Federal do Ceará - UFC (1) Quinderé, Ana Luíza Gomes; Universidade Federal do Ceará - UFC (1) Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia - RENORBIO (1) Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia - RENÓRBIO (1) Vanderlei, Edfranck de Sousa Oliveira; Universidade Federal do Ceará - UFC (1) Mais... Palavra-chave 2.08.01.00-9 Halymeniales (2) Anticoagulant activity (1) Anticoagulant activity 2.08.01.00-9 Halymeniales (1) Blood coagulation 2.08.01.00-9 (1) Drying methods (1) Halymenia floresia (1) Sulfated galactans (1) Sulfated macromolecules (1) Mais... Tipo do documento Bioprospecção (1) Ano 2011 (2) País Brazil (2) Idioma Inglês (1) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from Halymenia sp. (Rhodophyceae) - doi: 10.4025/actascibiolsci.v34i1.6961 Biological Sciences Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia - RENÓRBIO; Vanderlei, Edfranck de Sousa Oliveira; Universidade Federal do Ceará - UFC; Quinderé, Ana Luíza Gomes; Universidade Federal do Ceará - UFC; Queiroz, Ismael Nilo Lino de; Universidade Federal do Ceará - UFC; Bessa, Érika Façanha; Universidade Federal do Ceará - UFC; Benevides, Norma Maria Barros; Universidade Federal do Ceará - UFC. Sulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from Halymenia sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs) were first extracted with papain in 100 mM sodium acetate (pH 5.0) containing cysteine and EDTA (5 mM). The TSPs obtained were dried in an oven (M I) or lyophilized (M II) and then examined by ion exchange chromatography (DEAE-cellulose) using the NaCl gradient technique. The fractions were analyzed by 0.5% agarose gel electrophoresis and the in vitro anticoagulant activity was evaluated by the activated partial thromboplastin time test using normal... Palavras-chave: 2.08.01.00-9 Halymeniales; Sulfated macromolecules; Drying methods; Blood coagulation 2.08.01.00-9. Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6961 Anticoagulant properties of a crude sulfated polysaccharide from the red marine alga Halymenia floresia (Clemente) C. Agardh - doi: 10.4025/actascibiolsci.v33i3.6402 Biological Sciences Amorim, Rodrigo César das Neves; Universidade Federal da Amazônia; Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia - RENORBIO; Holanda, Márjory Lima; Universidade Federal do Maranhão; Mourão, Paulo Antônio de Souza; Universidade Federal do Rio de Janeiro; Benevides, Norma Maria Barros; Universidade Federal do Ceará. Alternative sources of anticoagulants have arisen as a result of the increasing demand for safer anticoagulant clinical therapy, and the sulfated polysaccharides of seaweeds have gained attention in biomedicine. In this study, crude sulfated polysaccharide fractions (denominated Hf1, Hf2 and Hf3) were obtained from the red marine alga Halymenia floresia and the anticoagulant properties of a soluble crude polysaccharide fraction (Hf2s) were assayed. The three differential extractions yielded 38.6%. The polysaccharides are composed mainly of galactose, with small amounts of xylose and glucose. The anticoagulant properties of Hf2s containing 53.8% sulfate and 3% protein was also compared to those of heparin (193.0 IU mg-1) by assays of activated partial... Tipo: Bioprospecção Palavras-chave: 2.08.01.00-9 Halymeniales; Halymenia floresia; Sulfated galactans; Anticoagulant activity 2.08.01.00-9 Halymeniales; Halymenia floresia; Sulfated galactans; Anticoagulant activity. Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6402 Registros recuperados: 2 Primeira ... 1 ... Última

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